摘要 色氨酸是人和动物必需的8种氨基酸之一。由于L-色氨酸特殊的结构和生物学功能，它在人和动物的生长发育及新陈代谢中有着至关重要的作用，因而被广泛应用于食品、饲料添加剂、医药、农业及环境监测等方面。随着人们对色氨酸的用途越来越了解，色氨酸的市场会迅速增加，前景乐观。L-色氨酸工业属于现代发酵工程，本设计是L-色氨酸的提取车间设计。以葡萄糖为原料，以大肠杆菌为生产菌株，采用发酵法生产L-色氨酸，经膜分离、离交、脱色、超滤、真空浓缩、结晶、干燥等工艺提取L-色氨酸。设计年产纯度为98.5%以上的结晶L-色氨酸4000吨，产酸为3.8%，装液量/罐容为75%，L-色氨酸总提取收率为81%，发酵周期34 h，每年生产320天。本设计就L-色氨酸的提取工艺流程、物料衡算、热量衡算、设备的选型、工厂布置、车间设计、水、电、气、汽以及辅助设备、三废处理及综合利用等方面对L-色氨酸提取车间进行设计。整个设计考虑了目前的经济状况和今后的发展需求，从节能角度选择设备且生产流程尽量保证机械化、自动化，同时提取做到技术先进，产品质量合格，车间布局规范合理。 关键词：L-色氨酸；发酵；离子交换；提取车间 Abstract Tryptopha
n is o
ne of the eight ki
nds of esse
ntial ami
no acids for huma
ns a
nd a
nimals. Due to the special structure a
nd biological fu
nctio
n of L- tryptopha
n, has a very importa
nt role i
n the growth a
nd developme
nt of huma
ns a
nd a
nimals as well as metabolic process, it is widely used i
n food a
nd feed additives i
ndustry, pharmaceuticals, agriculture a
nd e
nviro
nme
ntal mo
nitori
ng. As people become more a
nd more aware of the use of tryptopha
n, tryptopha
n market will i
ncrease rapidly, the outlook is optimistic. L-trptopha
n i
ndustry belo
ngs to the moder
n ferme
ntatio
n e
ngi
neeri
ng, this work is a L-trptopha
n extractio
n workshop desig
n, with oral glucose as raw material, usi
ng Escherichia coli as productio
n strai
n, the ferme
ntatio
n productio
n of L-trptopha
n, by membra
ne separatio
n, io
n excha
nge, decolorizatio
n, ultrafiltratio
n, vacuum co
nce
ntratio
n, crystallizatio
n, dryi
ng etc. steps extract L-trptopha
n. Desig
n of purity of crystallizatio
n of more tha
n 98.5% L-trptopha
n 4000 to
ns, acid productio
n rate was 3.8%, the volume of liquid / ta
nk capacity is 75%, the total yield of L-trptopha
n is 81%, the ferme
ntatio
n period is 34 h, 320 days for every year. Accordi
ng to L-trptopha
n extractio
n desig
n of process flow, pla
nt material a
nd heat bala
nce calculatio
n, equipme
nt selectio
n, process layout, workshop desig
n, water, electricity, gas, steam a
nd auxiliary equipme
nt a
nd other aspects for L-trptopha
n extractio
n workshop desig
n. This desig
n takes full accou
nt of curre
nt eco
nomic situatio
n a
nd future developme
nt
needs, chooses equipme
nts from the perspective of e
nergy-efficie
nt, productio
n processes to maximize mecha
nizatio
n, automatio
n level, while seeki
ng to tech
nically adva
nced, reliable quality, reaso
nable a
nd
normal layout. Key words: L-trptopha
n; Ferme
ntatio
n; Io
n excha
nge; Extractio
n pla
nt 目录 第一章前言1 第一节L-色氨酸的理化性质1 第二节L-色氨酸的应用1 第三节L-色氨酸国内市场分析及工业生产概况2 第四节立题背景3 第二章总体设计方案4 第一节设计参数4 第二节提取流程思路4 第三节厂区总体构想........................................................................... 5 第三章提取工段的工艺论证7 第一节提取方法及其选择7 第二节工艺流程及其论证7 第四章物料衡算及设备选型..............................................................19 第一节物料衡算与设备选型..............................................................19 第二节设备计算..............................................................................25 第三节提取车间主要设备一览表29 参考文献 致谢 第一章前言 第一节L-色氨酸的理化性质 色氨酸是人和动物生命活动中八种必需氨基酸之一，在人和动物的生长发育、新陈代谢起着重要作用，是第二必需氨基酸。在1902年，Hoki
nst第一次从酪蛋白中分离获得色氨酸。其英文名为L-Tryptopha
n(L-Trp)，学名为β-吲哚基丙氨酸，化学名称是L-2-氨基-3-吲哚基丙酸，别名为L-氨基吲哚丙酸、L-胰化蛋白氨基酸。分子式为C11H12O2N2，L-色氨酸的相对分子质量为204.21。